Determination of SARS‐coronavirus by a microfluidic chip system
Identifieur interne : 005912 ( Main/Exploration ); précédent : 005911; suivant : 005913Determination of SARS‐coronavirus by a microfluidic chip system
Auteurs : Xiaomian Zhou [République populaire de Chine] ; Dayu Liu [République populaire de Chine] ; Runtao Zhong [République populaire de Chine] ; Zhongpeng Dai [République populaire de Chine] ; Dapeng Wu [République populaire de Chine] ; Hui Wang [République populaire de Chine] ; Yuguang Du [République populaire de Chine] ; Zhinan Xia [États-Unis] ; Liping Zhang [États-Unis] ; Xiaodai Mei [République populaire de Chine] ; Bingcheng Lin [République populaire de Chine]Source :
- ELECTROPHORESIS [ 0173-0835 ] ; 2004-09.
Descripteurs français
- KwdFr :
- MESH :
- isolement et purification : Virus du SRAS.
- virologie : Poumon.
- Autopsie, Humains, Laboratoires sur puces, Procédures d'analyse sur micropuce, Réaction de polymérisation en chaîne, Électrophorèse capillaire.
English descriptors
- KwdEn :
- MESH :
- isolation & purification : SARS Virus.
- methods : Electrophoresis, Capillary, Microchip Analytical Procedures.
- virology : Lung.
- Teeft :
- Alternative methods, Amplification, Assay, Autopsy, Cdna, Chem, Chemical physics, Chinese academy, Clinical sars samples, Clinical specimens, Dalian institute, Detection limit, Detection system, Duplex, Early stage, Electrophoresis, Electrophoretic analysis, Electrophoretic sizing, Gmbh, High sensitivity, Humans, Intrareservoir temperature, Kgaa, Lab-On-A-Chip Devices, Medical university, Microchip, Microfluidic, Microfluidic chip, Microfluidic chip analyzer, Microfluidic chip system, Migration time, Molecular test, Nasopharyngeal swabs, Negative control, Nucleic acid stain, Parainfluenza virus, Peltier, Peltier surface temperature, Photomultiplier tube, Polymerase Chain Reaction, Positive control, Positive rate, Primer, Reaction mixture, Restriction fragments, Rnaeasy mini, Same chip, Same time, Sample reservoir, Sample waste, Sample waste reservoirs, Sars, Sars genome sequence, Sars patients, Takara biotechnology, Thermal cycler, Verlag, Verlag gmbh, Virus isolation, Waste reservoirs, Weinheim, Weinheim figure.
Abstract
We have developed a new experimental system based on a microfluidic chip to determine severe acute respiratory syndrome coronavirus (SARS‐CoV). The system includes a laser‐induced fluorescence microfluidic chip analyzer, a glass microchip for both polymerase chain reaction (PCR) and capillary electrophoresis, a chip thermal cycler based on dual Peltier thermoelectric elements, a reverse transcription‐polymerase chain reaction (RT‐PCR) SARS diagnostic kit, and a DNA electrophoretic sizing kit. The system allows efficient cDNA amplification of SARS‐CoV followed by electrophoretic sizing and detection on the same chip. To enhance the reliability of RT‐PCR on SARS‐CoV detection, duplex PCR was developed on the microchip. The assay was carried out on a home‐made microfluidic chip system. The positive and the negative control were cDNA fragments of SARS‐CoV and parainfluenza virus, respectively. The test results showed that 17 positive samples were obtained among 18 samples of nasopharyngeal swabs from clinically diagnosed SARS patients. However, 12 positive results from the same 18 samples were obtained by the conventional RT‐PCR with agarose gel electrophoresis detection. The SARS virus species can be analyzed with high positive rate and rapidity on the microfluidic chip system.
Url:
DOI: 10.1002/elps.200305966
Affiliations:
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last="Wu">Dapeng Wu</name><affiliation wicri:level="1"><country xml:lang="fr">République populaire de Chine</country><wicri:regionArea>Dalian Institute of Chemical Physics, Chinese Academy of Sciences, Dalian</wicri:regionArea><wicri:noRegion>Dalian</wicri:noRegion></affiliation></author><author><name sortKey="Wang, Hui" sort="Wang, Hui" uniqKey="Wang H" first="Hui" last="Wang">Hui Wang</name><affiliation wicri:level="1"><country xml:lang="fr">République populaire de Chine</country><wicri:regionArea>Dalian Institute of Chemical Physics, Chinese Academy of Sciences, Dalian</wicri:regionArea><wicri:noRegion>Dalian</wicri:noRegion></affiliation></author><author><name sortKey="Du, Yuguang" sort="Du, Yuguang" uniqKey="Du Y" first="Yuguang" last="Du">Yuguang Du</name><affiliation wicri:level="1"><country xml:lang="fr">République populaire de Chine</country><wicri:regionArea>Dalian Institute of Chemical Physics, Chinese Academy of Sciences, 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wicri:level="1"><country xml:lang="fr">République populaire de Chine</country><wicri:regionArea>Dalian Institute of Chemical Physics, Chinese Academy of Sciences, Dalian</wicri:regionArea><wicri:noRegion>Dalian</wicri:noRegion></affiliation></author><author><name sortKey="Lin, Bingcheng" sort="Lin, Bingcheng" uniqKey="Lin B" first="Bingcheng" last="Lin">Bingcheng Lin</name><affiliation wicri:level="1"><country xml:lang="fr">République populaire de Chine</country><wicri:regionArea>Dalian Institute of Chemical Physics, Chinese Academy of Sciences, Dalian</wicri:regionArea><wicri:noRegion>Dalian</wicri:noRegion></affiliation><affiliation wicri:level="1"><country wicri:rule="url">République populaire de Chine</country></affiliation><affiliation></affiliation></author></analytic><monogr></monogr><series><title level="j" type="main">ELECTROPHORESIS</title><title level="j" type="alt">ELECTROPHORESIS</title><idno type="ISSN">0173-0835</idno><idno type="eISSN">1522-2683</idno><imprint><biblScope unit="vol">25</biblScope><biblScope unit="issue">17</biblScope><biblScope unit="page" from="3032">3032</biblScope><biblScope unit="page" to="3039">3039</biblScope><biblScope unit="page-count">8</biblScope><publisher>WILEY‐VCH Verlag</publisher><pubPlace>Weinheim</pubPlace><date type="published" when="2004-09">2004-09</date></imprint><idno type="ISSN">0173-0835</idno></series></biblStruct></sourceDesc><seriesStmt><idno type="ISSN">0173-0835</idno></seriesStmt></fileDesc><profileDesc><textClass><keywords scheme="KwdEn" xml:lang="en"><term>Autopsy</term><term>Electrophoresis, Capillary (methods)</term><term>Humans</term><term>Lab-On-A-Chip Devices</term><term>Lung (virology)</term><term>Microchip Analytical Procedures (methods)</term><term>Polymerase Chain Reaction</term><term>SARS Virus (isolation & purification)</term></keywords><keywords scheme="KwdFr" xml:lang="fr"><term>Autopsie</term><term>Humains</term><term>Laboratoires sur puces</term><term>Poumon (virologie)</term><term>Procédures d'analyse sur micropuce ()</term><term>Réaction de polymérisation en chaîne</term><term>Virus du SRAS (isolement et purification)</term><term>Électrophorèse capillaire ()</term></keywords><keywords scheme="MESH" qualifier="isolation & purification" xml:lang="en"><term>SARS Virus</term></keywords><keywords scheme="MESH" qualifier="isolement et purification" xml:lang="fr"><term>Virus du SRAS</term></keywords><keywords scheme="MESH" qualifier="methods" xml:lang="en"><term>Electrophoresis, Capillary</term><term>Microchip Analytical Procedures</term></keywords><keywords scheme="MESH" qualifier="virologie" xml:lang="fr"><term>Poumon</term></keywords><keywords scheme="MESH" qualifier="virology" xml:lang="en"><term>Lung</term></keywords><keywords scheme="Teeft" xml:lang="en"><term>Alternative methods</term><term>Amplification</term><term>Assay</term><term>Autopsy</term><term>Cdna</term><term>Chem</term><term>Chemical physics</term><term>Chinese academy</term><term>Clinical sars samples</term><term>Clinical specimens</term><term>Dalian institute</term><term>Detection limit</term><term>Detection system</term><term>Duplex</term><term>Early stage</term><term>Electrophoresis</term><term>Electrophoretic analysis</term><term>Electrophoretic sizing</term><term>Gmbh</term><term>High sensitivity</term><term>Humans</term><term>Intrareservoir temperature</term><term>Kgaa</term><term>Lab-On-A-Chip Devices</term><term>Medical university</term><term>Microchip</term><term>Microfluidic</term><term>Microfluidic chip</term><term>Microfluidic chip analyzer</term><term>Microfluidic chip system</term><term>Migration time</term><term>Molecular test</term><term>Nasopharyngeal swabs</term><term>Negative control</term><term>Nucleic acid stain</term><term>Parainfluenza virus</term><term>Peltier</term><term>Peltier surface temperature</term><term>Photomultiplier tube</term><term>Polymerase Chain Reaction</term><term>Positive control</term><term>Positive rate</term><term>Primer</term><term>Reaction mixture</term><term>Restriction fragments</term><term>Rnaeasy mini</term><term>Same chip</term><term>Same time</term><term>Sample reservoir</term><term>Sample waste</term><term>Sample waste reservoirs</term><term>Sars</term><term>Sars genome sequence</term><term>Sars patients</term><term>Takara biotechnology</term><term>Thermal cycler</term><term>Verlag</term><term>Verlag gmbh</term><term>Virus isolation</term><term>Waste reservoirs</term><term>Weinheim</term><term>Weinheim figure</term></keywords><keywords scheme="MESH" xml:lang="fr"><term>Autopsie</term><term>Humains</term><term>Laboratoires sur puces</term><term>Procédures d'analyse sur micropuce</term><term>Réaction de polymérisation en chaîne</term><term>Électrophorèse capillaire</term></keywords></textClass></profileDesc></teiHeader><front><div type="abstract" xml:lang="en">We have developed a new experimental system based on a microfluidic chip to determine severe acute respiratory syndrome coronavirus (SARS‐CoV). The system includes a laser‐induced fluorescence microfluidic chip analyzer, a glass microchip for both polymerase chain reaction (PCR) and capillary electrophoresis, a chip thermal cycler based on dual Peltier thermoelectric elements, a reverse transcription‐polymerase chain reaction (RT‐PCR) SARS diagnostic kit, and a DNA electrophoretic sizing kit. The system allows efficient cDNA amplification of SARS‐CoV followed by electrophoretic sizing and detection on the same chip. To enhance the reliability of RT‐PCR on SARS‐CoV detection, duplex PCR was developed on the microchip. The assay was carried out on a home‐made microfluidic chip system. The positive and the negative control were cDNA fragments of SARS‐CoV and parainfluenza virus, respectively. The test results showed that 17 positive samples were obtained among 18 samples of nasopharyngeal swabs from clinically diagnosed SARS patients. However, 12 positive results from the same 18 samples were obtained by the conventional RT‐PCR with agarose gel electrophoresis detection. The SARS virus species can be analyzed with high positive rate and rapidity on the microfluidic chip system.</div></front></TEI><affiliations><list><country><li>République populaire de Chine</li><li>États-Unis</li></country><region><li>Massachusetts</li></region></list><tree><country name="République populaire de Chine"><noRegion><name sortKey="Zhou, Xiaomian" sort="Zhou, Xiaomian" uniqKey="Zhou X" first="Xiaomian" last="Zhou">Xiaomian Zhou</name></noRegion><name sortKey="Dai, Zhongpeng" sort="Dai, Zhongpeng" uniqKey="Dai Z" first="Zhongpeng" last="Dai">Zhongpeng Dai</name><name sortKey="Du, Yuguang" sort="Du, Yuguang" uniqKey="Du Y" first="Yuguang" last="Du">Yuguang Du</name><name sortKey="Lin, Bingcheng" sort="Lin, Bingcheng" uniqKey="Lin B" first="Bingcheng" last="Lin">Bingcheng Lin</name><name sortKey="Lin, Bingcheng" sort="Lin, Bingcheng" uniqKey="Lin B" first="Bingcheng" last="Lin">Bingcheng Lin</name><name sortKey="Liu, Dayu" sort="Liu, Dayu" uniqKey="Liu D" first="Dayu" last="Liu">Dayu Liu</name><name sortKey="Mei, Xiaodai" sort="Mei, Xiaodai" uniqKey="Mei X" first="Xiaodai" last="Mei">Xiaodai Mei</name><name sortKey="Wang, Hui" sort="Wang, Hui" uniqKey="Wang H" first="Hui" last="Wang">Hui Wang</name><name sortKey="Wu, Dapeng" sort="Wu, Dapeng" uniqKey="Wu D" first="Dapeng" last="Wu">Dapeng Wu</name><name sortKey="Zhong, Runtao" sort="Zhong, Runtao" uniqKey="Zhong R" first="Runtao" last="Zhong">Runtao Zhong</name></country><country name="États-Unis"><region name="Massachusetts"><name sortKey="Xia, Zhinan" sort="Xia, Zhinan" uniqKey="Xia Z" first="Zhinan" last="Xia">Zhinan Xia</name></region><name sortKey="Zhang, Liping" sort="Zhang, Liping" uniqKey="Zhang L" first="Liping" last="Zhang">Liping Zhang</name></country></tree></affiliations></record>Pour manipuler ce document sous Unix (Dilib)
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